CD147 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82909-7-RR (same clone as 82909-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82909-7-RR (CD147 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82909-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82909-7-RR against Human CD147 were performed. The affinity constant is 21.7 nM.

CD147 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82909-7-PBS

Clone No.230046A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7

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Product Information

82909-7-PBS targets CD147 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: basigin (Ok blood group)
• Calculated Molecular Weight: 385 aa, 42 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC009040
• Gene Symbol: CD147
• Gene ID (NCBI): 682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35613
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).