CoraLite®555-conjugated CD146/MCAM Monoclonal antibody

CD146/MCAM Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4D8A9

Cat no : CL555-66153

Synonyms

CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

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Validation Data Gallery

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  IF Staining of human liver cancer using CL555-66153

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 CD146/MCAM antibody (CL555-66153, Clone: 4D8A9 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL555-66153

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 CD146/MCAM antibody (CL555-66153, Clone: 4D8A9 ) at dilution of 1:200.

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  IF Staining of HUVEC using CL555-66153

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®555 CD146/MCAM antibody (CL555-66153, Clone: 4D8A9 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66153 targets CD146/MCAM in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD146/MCAM fusion protein Ag11855
• Full Name: melanoma cell adhesion molecule
• Calculated Molecular Weight: 646 aa, 72 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC056418
• Gene Symbol: CD146
• Gene ID (NCBI): 4162
• RRID: AB_2919675
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.