CD141/Thrombomodulin Recombinant antibody

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  WB analysis of mouse placenta using 84412-3-RR

  mouse placenta tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 84412-3-RR

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of bEnd.3 using 84412-3-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD141/Thrombomodulin antibody (84412-3-RR, Clone: 241730D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84412-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84412-3-RR against Mouse CD141/Thrombomodulin were performed. The affinity constant is 3.11 nM.

CD141/Thrombomodulin Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84412-3-RR

Clone No.241730D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

TM, Thrombomodulin, Thbd, Fetomodulin, CD141

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Tested Applications

• Positive WB detected in: mouse placenta tissue, mouse lung tissue
• Positive IF/ICC detected in: bEnd.3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84412-3-RR targets CD141/Thrombomodulin in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: thrombomodulin
• Calculated Molecular Weight: 62kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_009378.3
• Gene Symbol: Thbd
• Gene ID (NCBI): 21824
• RRID: AB_3671941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15306
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).