Mouse CD14 Recombinant antibody, PBS Only

CD14 Recombinant Antibody for IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240225F1

Cat no : 83297-5-PBS

Synonyms

Cd14, CD14 antigen

---

Validation Data Gallery

• 

  IF Staining of RAW 264.7 using 83297-5-RR (same clone as 83297-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-5-RR, Clone: 240225F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83297-5-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 83297-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83297-5-RR against Mouse CD14 were performed. The affinity constant is 0.961 nM.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83297-5-PBS is CD14 Fusion Protein expressed in E. coli.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.