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Species Reactivity
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Conjugate
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Conjugate
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CD14 Recombinant antibody

CD14 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240225F1

Cat no : 83297-5-RR

Synonyms

CD14 antigen, 240225F1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Recombinant antibody (83297-5-RR)

    IF Staining of RAW 264.7 using 83297-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-5-RR, Clone: 240225F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83297-5-RR

    Affinity and Kinetic Characterization of 83297-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83297-5-RR against Mouse CD14 were performed. The affinity constant is 0.248 nM.

Tested Applications

Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83297-5-RR is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD14 antigen
Calculated Molecular Weight39 kDa
GenBank Accession NumberNM_009841.4
Gene Symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
IF protocol for CD14 antibody 83297-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols