Mouse CD14 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00313-4

  Sandwich ELISA standard curve of MP00313-4, Mouse CD14 Monoclonal Matched Antibody Pair - PBS only. 83297-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0847. 83297-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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  Sample test of MP00313-4

  Mouse splenocytes (1 x 10^6 cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS.The mean CD-14 concentration was determined to be 2,187.4 pg/mL in unstimulated spleen, 8,939.2 pg/mL in IFN-γ and LPS stimulated spleen supernatant.

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  Sample test of MP00313-4

  Serum of sixteen mice was measured. The CD14 concentration of detected samples was determined to be 113.29 ng/mL with a range of 83.24-160.50 ng/mL.

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  Cytometric bead array standard curve of MP00313-1

  Cytometric bead array standard curve of MP00313-1, MOUSE CD14 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83297-3-PBS. Detection antibody: 83297-1-PBS. Standard: Eg0847. Range: 1.25-40 ng/mL.

CD14 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83297-1-PBS

Clone No.240225A8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD14 antigen, 240225A8

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Product Information

83297-1-PBS targets CD14 as part of a matched antibody pair:

MP00313-1: 83297-3-PBS capture and 83297-1-PBS detection (validated in Cytometric bead array)

MP00313-4: 83297-7-PBS capture and 83297-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10810
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.