CD14 Recombinant antibody, PBS Only

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  WB analysis of HL-60 using 83055-6-RR (same clone as 83055-6-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 83055-6-RR (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83055-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83055-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83055-6-RR against Human CD14 were performed. The affinity constant is 1.43 nM.

CD14 {ptg:Brand} Recombinant Antibody for WB, Indirect ELISA

Cat No. 83055-6-PBS

Clone No.230332D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D4

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Product Information

83055-6-PBS targets CD14 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• ENSEMBL Gene ID: ENSG00000170458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08571
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.