CD14 Polyclonal antibody

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  WB analysis using 31119-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31119-1-AP (CD14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CD14 Polyclonal Antibody for WB, ELISA

Cat No. 31119-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: J774A.1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31119-1-AP targets CD14 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10810
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.