CD138/Syndecan-1 Monoclonal antibody, PBS Only (Detector)

CD138/Syndecan-1 Monoclonal Antibody for IF, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F3A8

Cat no : 67155-1-PBS

Synonyms

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50044-1

  Sandwich ELISA standard curve of MP50044-1, SDC1,CD138 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60185-1-PBS. Detection antibody: 67155-1-PBS. Standard: Ag17733. Range: 0.188-12 ng/mL.

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  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67155-1-PBS targets CD138/Syndecan-1 as part of a matched antibody pair:

MP50044-1: 60185-1-PBS capture and 67155-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Full Name: syndecan 1
• Calculated Molecular Weight: 32.5 kDa
• GenBank Accession Number: BC008765
• Gene Symbol: SDC1
• Gene ID (NCBI): 6382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.