CD138/Syndecan-1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD138/Syndecan-1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10593-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10593-1-AP (same clone as 10593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 10593-1-AP (same clone as 10593-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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Product Information

10593-1-PBS targets CD138/Syndecan-1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Full Name: syndecan 1
• Calculated Molecular Weight: 32.5 kDa
• Observed Molecular Weight: 60-100 kDa, 130 kDa
• GenBank Accession Number: BC008765
• Gene Symbol: CD138
• Gene ID (NCBI): 6382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.