Anti-Human TNFRSF9/CD137 (4B4-1)

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  FC experiment of human peripheral blood mononuclear cells using 65159-1-Ig

  1X10^6 PHA stimulated (5 μg/mL, 3 days) (right) or unstimulated (left) human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.13 ug Anti-Human CD137 (65159-1-Ig, Clone: 4B4-1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

TNFRSF9/CD137 Monoclonal Antibody for FC

Cat No. 65159-1-Ig

Clone No.4B4-1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Synonyms

TNFRSF9, 4 1BB, 4 1BB ligand receptor, 4-1BB ligand receptor, 4B4-1

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Tested Applications

• Positive FC detected in: human peripheral blood mononuclear cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65159-1-Ig targets TNFRSF9/CD137 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Recombinant human CD137 ectodomain

• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC006196
• Gene Symbol: TNFRSF9/CD137
• Gene ID (NCBI): 3604
• RRID: AB_2918441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: Q07011
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)