CD122/IL-2RB Monoclonal antibody, PBS Only (Detector)

CD122/IL-2RB Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12C7

Cat no : 60174-1-PBS

Synonyms

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

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Validation Data Gallery

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  WB analysis using 60174-1-Ig (same clone as 60174-1-PBS)

  Untreated and PNGase F-treated lysates of NK-92 cells were subjected to SDS PAGE followed by western blot with 60174-1-Ig (CD122/IL2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-1-PBS in a different storage buffer formulation.

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  WB analysis of Recombinant protein using 60174-1-Ig (same clone as 60174-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-1-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50451-1

  Cytometric bead array standard curve of MP50451-1, CD122/IL2RB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60174-3-PBS. Detection antibody: 60174-1-PBS. Standard:Ag3929. Range: 0.098-25 ng/mL.

• 

  Cytometric bead array standard curve of MP50451-2

  Cytometric bead array standard curve of MP50451-2, CD122/IL2RB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60174-2-PBS. Detection antibody: 60174-1-PBS. Standard:Ag3929. Range: 0.098-50 ng/mL.

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Product Information

60174-1-PBS targets CD122/IL-2RB as part of a matched antibody pair:

MP50451-1: 60174-3-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)

MP50451-2: 60174-2-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD122/IL-2RB fusion protein Ag3929
• Full Name: interleukin 2 receptor, beta
• Calculated Molecular Weight: 551 aa, 61 kDa
• Observed Molecular Weight: 75 kDa, 61 kDa
• GenBank Accession Number: BC025691
• Gene Symbol: IL2RB
• Gene ID (NCBI): 3560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.