CD11c/Integrin Alpha X Monoclonal antibody, PBS Only

CD11c/Integrin Alpha X Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F1C10

Cat no : 60258-1-PBS

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

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Validation Data Gallery

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  WB analysis of HL-60 using 60258-1-Ig (same clone as 60258-1-PBS)

  Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 60258-1-Ig (same clone as 60258-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 60258-1-Ig (same clone as 60258-1-PBS)

  Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 60258-1-Ig (same clone as 60258-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 60258-1-Ig (same clone as 60258-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60258-1-Ig (same clone as 60258-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60258-1-PBS targets CD11c/Integrin Alpha X in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD11c/Integrin Alpha X fusion protein Ag11350
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.