Anti-Human CD11c (BU15) Mouse IgG2a Recombinant Antibody, PBS Only

CD11c Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU15

Cat no : 65600-1-PBS

Synonyms

Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocyte using 65600-1-MR (same clone as 65600-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65600-1-MR, Clone: BU15) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65600-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 65600-1-MR (same clone as 65600-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65600-1-MR, Clone: BU15) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65600-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 65600-1-MR (same clone as 65600-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65600-1-MR, Clone: BU15) (left) or 0.25 ug Anti-Human CD11c (65196-1-Ig, Clone: BU15) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65600-1-PBS in a different storage buffer formulation.

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Product Information

65600-1-PBS targets CD11c in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human synovial fluid dendritic cells
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).