CD11a/Integrin Alpha L Monoclonal antibody, PBS Only

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  WB analysis of Ramos using 66256-1-Ig (same clone as 66256-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

CD11a/Integrin Alpha L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66256-1-PBS

Clone No.1E3H12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CD11a, ITGAL, Integrin alpha-L, Integrin alpha L, CD11 antigen-like family member A

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Product Information

66256-1-PBS targets CD11a/Integrin Alpha L in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD11a/Integrin Alpha L fusion protein Ag7813
• Full Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 129 kDa
• GenBank Accession Number: BC008777
• Gene Symbol: CD11a
• Gene ID (NCBI): 3683
• ENSEMBL Gene ID: ENSG00000005844
• RRID: AB_2881644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20701
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).