Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody, PBS Only

CD11a Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/4

Cat no : 65563-1-PBS

Synonyms

Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocyte using 65563-1-MR (same clone as 65563-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11a Mouse IgG2a Recombinant Antibody (65563-1-MR, Clone: TS2/4) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65563-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 65563-1-MR (same clone as 65563-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11a Mouse IgG2a Recombinant Antibody (65563-1-MR, Clone: TS2/4) (left) or 0.25 ug Anti-Human CD11a (65194-1-Ig, Clone: TS2/4) (right), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65563-1-PBS in a different storage buffer formulation.

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Product Information

65563-1-PBS targets CD11a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DR CTL line
• Full Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 129 kDa
• GenBank Accession Number: BC008777
• Gene Symbol: CD11a
• Gene ID (NCBI): 3683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).