FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65518-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65518-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65518-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.
FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
65518-1-PBS targets CD11a in FC applications and shows reactivity with human samples.
Store at -80°C. Stable for one year after shipment.
Background Information
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65518-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65518-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65518-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.
FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD11a Recombinant antibody
Catalog Number
65518-1-PBS
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
