PE Anti-Mouse CD117/c-Kit (ACK2)

CD117/c-Kit Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

ACK2

Cat no : PE-65094

Synonyms

Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using PE-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug APC-Anti-Mouse CD11b (APC-65055, clone M1/70) and 0.20 ug PE-Anti-Mouse CD117 (c-Kit) (PE-65094, clone ACK2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using PE-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug APC-Anti-Mouse CD11b (APC-65055, clone M1/70) and 0.20 ug PE-Rat IgG2b isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65094 targets CD117/c-Kit in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Murine IL-3 dependent mast cells
• Full Name: kit oncogene
• GenBank Accession Number: BC052457
• Gene Symbol: Kit
• Gene ID (NCBI): 16590
• RRID: AB_2883888
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).