CD107a / LAMP1 Recombinant antibody, PBS Only

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  WB analysis using 84658-5-RR (same clone as 84658-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84658-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84658-5-RR (same clone as 84658-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD107a / LAMP1 antibody (84658-5-RR, Clone: 242116E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84658-5-PBS in a different storage buffer formulation.

CD107a / LAMP1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84658-5-PBS

Clone No.242116E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CD107a, LAMP1, 242116E8, CD107 antigen-like family member A, CD107a/LAMP1

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Product Information

84658-5-PBS targets CD107a / LAMP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD107a / LAMP1 fusion protein Eg2611
• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: NM_005561.4
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P11279-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).