Anti-Mouse Endoglin/CD105 (MJ7/18)

Endoglin/CD105 Monoclonal Antibody for IF-P, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-P, FC

Conjugate

Unconjugated

CloneNo.

MJ7/18

Cat no : 65048-1-Ig

Synonyms

CD105, S endoglin, MJ7/18, Eng, endoglin

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Validation Data Gallery

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  IF Staining of mouse liver using 65048-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD105 antibody (65048-1-Ig, Clone: MJ7/18 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of bEnd.3 using 65048-1-Ig

  1X10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse CD105 (65048-1-Ig, Clone:MJ7/18) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive IF-P detected in: mouse liver tissue
• Positive FC detected in: bEnd.3 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65048-1-Ig targets Endoglin/CD105 in IF-P, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Inflamed mouse skin
• Full Name: endoglin
• GenBank Accession Number: BC029080
• Gene Symbol: CD105
• Gene ID (NCBI): 13805
• RRID: AB_2918360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)