Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse and More (1)

Applications

IF, FC

Conjugate

Unconjugated

Publications

3

CloneNo.

2E7

Cat no : 65047-1-Ig

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Integrin alpha E, Integrin alpha M290, Itgae

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse splenocytes using 65047-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD103 (65047-1-Ig, Clone:2E7) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100, or 0.5 ug Armenian Hamster IgG isotype control and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• FC: See 1 publications below

Product Information

65047-1-Ig targets CD103 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
• Full Name: integrin alpha E, epithelial-associated
• GenBank Accession Number: BC150690
• Gene Symbol: CD103
• Gene ID (NCBI): 16407
• RRID: AB_2918359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

Publications

Species	Application	Title
mouse	IF	Biomolecules; Oral Immunization with Escherichia coli Nissle 1917 Expressing SARS-CoV-2 Spike Protein Induces Mucosal and Systemic Antibody Responses in Mice; Authors - Giovanni Sarnelli
mouse	FC	J Nanobiotechnology; Optogenetic engineered umbilical cord MSC-derived exosomes for remodeling of the immune microenvironment in diabetic wounds and the promotion of tissue repair; Authors - Xin Zhao
	IF	Biomed Pharmacother; Intranasal administration of Escherichia coli Nissle expressing the spike protein of SARS-CoV-2 induces long-term immunization and prevents spike protein-mediated lung injury in mice; Authors - Giovanni Sarnelli