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CD100 Recombinant antibody, PBS Only

CD100 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241708D10

Cat no : 84465-4-PBS

Synonyms

SEMA4D, SEM4D, CD100 antigen, CD 100, A8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD100 Recombinant antibody (84465-4-RR)

    IF Staining of Jurkat using 84465-4-RR (same clone as 84465-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD100 antibody (84465-4-RR, Clone: 241708D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84465-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84465-4-RR

    Affinity and Kinetic Characterization of 84465-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84465-4-RR against Human CD100 were performed. The affinity constant is 3.21 nM.

Product Information

84465-4-PBS targets CD100 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated Molecular Weight 96 kDa
GenBank Accession NumberBC054500
Gene Symbol SEMA4D/CD100
Gene ID (NCBI) 10507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.