CD100 Polyclonal antibody, PBS Only

CD100 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27516-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 27516-1-AP (same clone as 27516-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27516-1-AP (CD100 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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Product Information

27516-1-PBS targets CD100 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD100 fusion protein Ag26461
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC054500
• Gene Symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.