Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody, PBS Only

Neprilysin/CD10 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241322E1

Cat no : 98116-1-PBS

Synonyms

MME, MME,CD10, Enkephalinase, EC:3.4.24.11, CD10

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98116-1-RR (same clone as 98116-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98116-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Reh using 98116-1-RR (same clone as 98116-1-PBS)

  1x10^6 Reh cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98116-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98116-1-RR (same clone as 98116-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone:241322E1)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98116-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98116-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98116-1-RR against Human CD10 were performed. The affinity constant is 9.9 nM.

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Product Information

98116-1-PBS targets Neprilysin/CD10 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• GenBank Accession Number: BC101658
• Gene Symbol: CD10
• Gene ID (NCBI): 4311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).