CCT8 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCT8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 12263-1-AP

Synonyms

D21S246, Chaperonin containing T-complex polypeptide 1 subunit 8, CCT-theta, CCTQ, CCT theta

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Validation Data Gallery

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  WB analysis using 12263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12263-1-AP

  WB result of CCT8 antibody (12263-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells.

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  IP experiment of Jurkat using 12263-1-AP

  IP result of anti-CCT8 (IP:12263-1-AP, 4ug; Detection:12263-1-AP 1:500) with Jurkat cells lysate 3160 ug.

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  IF Staining of SW480 using 12263-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CCT8 antibody (12263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 12263-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A431 cells, mouse kidney tissue, HEK-293 cells, COLO 320 cells, HeLa cells, Jurkat cells, Raji cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: SW480 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12263-1-AP targets CCT8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCT8 fusion protein Ag2926
• Full Name: chaperonin containing TCP1, subunit 8 (theta)
• Calculated Molecular Weight: 548 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012584
• Gene Symbol: CCT8
• Gene ID (NCBI): 10694
• RRID: AB_906331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638)

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
mouse	WB	Neuron; Network organization of the huntingtin proteomic interactome in mammalian brain.; Authors - Dyna I Shirasaki
human	WB	Nat Commun; Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.; Authors - Alireza Noormohammadi
human	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
human	WB	PLoS One; TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.; Authors - Somalinga Balajee R BR

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

CCT8 band shows up prominently with no background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

CCT8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:12263-1-AP)