CCT5 Recombinant antibody

CCT5 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241510B3

Cat no : 84310-2-RR

Synonyms

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Validation Data Gallery

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  WB analysis using 84310-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84310-2-RR (CCT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells, HeLa cells, Jurkat cells, A549 cells, COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84310-2-RR is CCT5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCT5 fusion protein Ag2146
• Full Name: chaperonin containing TCP1, subunit 5 (epsilon)
• Calculated Molecular Weight: 541 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035499
• Gene Symbol: CCT5
• Gene ID (NCBI): 22948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.