CoraLite® Plus 488-conjugated CCT4 Monoclonal antibody

CCT4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C6A6

Cat no : CL488-67455

Synonyms

Stimulator of TAR RNA-binding, SRB, Chaperonin containing T-complex polypeptide 1 subunit 4, CCT-delta, CCTD

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Validation Data Gallery

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  IF Staining of U2OS using CL488-67455

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 CCT4 antibody (CL488-67455, Clone: 3C6A6 ) at dilution of 1:200.

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  FC experiment of A549 using CL488-67455

  1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cct4 Monoclonal Antibody (CL488-67455, Clone:3C6A6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67455 targets CCT4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig, rabbit samples.

• Tested Reactivity: human, mouse, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT4 fusion protein Ag17084
• Full Name: chaperonin containing TCP1, subunit 4 (delta)
• Calculated Molecular Weight: 539 aa, 58 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC106934
• Gene Symbol: CCT4
• Gene ID (NCBI): 10575
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.