Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 10571-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 10571-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10571-1-AP
IP result of anti-CCT3 (IP:10571-1-AP, 3ug; Detection:10571-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
IP result of anti-CCT3 (IP:10571-1-AP, 3ug; Detection:10571-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
IHC staining of human liver cancer using 10571-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10571-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 10571-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 10571-1-AP
Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of HepG2 using 10571-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, rat kidney tissue, HeLa cells, Jurkat cells, U2OS cells, mouse brain tissue, rat brain tissue, mouse kidney tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 10571-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 10571-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 10571-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10571-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse brain using 10571-1-AP
IP result of anti-CCT3 (IP:10571-1-AP, 3ug; Detection:10571-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
IF/ICC Figures
IF Staining of HepG2 using 10571-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 10571-1-AP
Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC (INTRA) Figures
FC experiment of HepG2 using 10571-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CCT3 Polyclonal antibody
Catalog Number
10571-1-AP
Citations
14
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
