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  • KD/KO Validated

CCT3 Polyclonal antibody

CCT3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10571-1-AP

Synonyms

TCP 1 gamma, hTRiC5, Chaperonin containing T-complex polypeptide 1 subunit 3, CCT-gamma, CCTgamma



Tested Applications

Positive WB detected inHEK-293 cells, rat kidney tissue, HeLa cells, Jurkat cells, U2OS cells, mouse brain tissue, rat brain tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10571-1-AP targets CCT3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT3 fusion protein Ag0843
Full Name chaperonin containing TCP1, subunit 3 (gamma)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC006501
Gene Symbol CCT3
Gene ID (NCBI) 7203
RRIDAB_2073658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCT3 antibody 10571-1-APDownload protocol
IHC protocol for CCT3 antibody 10571-1-APDownload protocol
IF protocol for CCT3 antibody 10571-1-APDownload protocol
IP protocol for CCT3 antibody 10571-1-APDownload protocol
FC protocol for CCT3 antibody 10571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Genet

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Authors - Tarkar Aarti A
mouseWB

Cell

Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.

Authors - Erik McShane
human,mouseWB

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S
  • KD Validated
humanWB

J Cell Biol

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Authors - Yu-Cheng Zhang
humanWB,IF

Dev Biol

Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae.

Authors - Redgrove Kate A KA
humanWB

Toxicol Appl Pharmacol

Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1.

Authors - Hongyang Shi
  • KD Validated