Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF Staining of NIH/3T3 using 68214-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)
Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF/ICC Figures
IF Staining of NIH/3T3 using 68214-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CCT2 Monoclonal antibody
Catalog Number
68214-1-Ig
Citations
1
Dilutions
WB : 1:5000-1:50000 IF/ICC : 1:200-1:800
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, pig, rabbit, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
