|Positive WB detected in||HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||mouse brain tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
22802-1-AP targets CCS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCS fusion protein Ag18850|
|Full Name||copper chaperone for superoxide dismutase|
|Calculated molecular weight||274 aa, 29 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC105016|
|Gene ID (NCBI)||9973|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.
|Product Specific Protocols|
|WB protocol for CCS antibody 22802-1-AP||Download protocol|
|IHC protocol for CCS antibody 22802-1-AP||Download protocol|
|IF protocol for CCS antibody 22802-1-AP||Download protocol|
|IP protocol for CCS antibody 22802-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Stem Cell
Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.
The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.
S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.
Br J Cancer
AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy
Mol Med Rep
Artemether ameliorates adriamycin induced cardiac atrophy in mice