CCS Polyclonal antibody

CCS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 22802-1-AP

Synonyms

Superoxide Dismutase 4

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Validation Data Gallery

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  WB analysis of HepG2 using 22802-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22802-1-AP (CCS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22802-1-AP

  IP result of anti-CCS (IP:22802-1-AP, 3ug; Detection:22802-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of mouse brain using 22802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22802-1-AP (CCS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22802-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22802-1-AP (CCS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22802-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22802-1-AP (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22802-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCS antibody (22802-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse brain tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

22802-1-AP targets CCS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCS fusion protein Ag18850
• Full Name: copper chaperone for superoxide dismutase
• Calculated Molecular Weight: 274 aa, 29 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC105016
• Gene Symbol: CCS
• Gene ID (NCBI): 9973
• RRID: AB_2879172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
human	WB	Neurobiol Dis; The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.; Authors - Nadine Bakkar
mouse	WB	Sci Rep; S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.; Authors - Sarah E Antinone
human	WB	Br J Cancer; AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy; Authors - Xiaomei Zhang
mouse	WB	Mol Med Rep; Artemether ameliorates adriamycin induced cardiac atrophy in mice; Authors - Wenci Weng
human	IHC	Clin Med Insights Oncol; Copper Chaperone for Superoxide Dismutase Subtypes as a Prognostic Marker in Luminal B Breast Cancer; Authors - Yanping Li