CCRL2 Monoclonal antibody

CCRL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2C1E11

Cat no : 66611-1-Ig

Synonyms

C C chemokine receptor like 2, CCR11, CCR6, CCRL2, Chemokine receptor CCR11, Chemokine receptor X, CKRX, CRAM, CRAM A, CRAM B, HCR

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Validation Data Gallery

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  WB analysis of HeLa using 66611-1-Ig

  HeLa cells and A431 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66611-1-Ig

  A431 cells and K-562 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 66611-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 66611-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

66611-1-Ig targets CCRL2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCRL2 fusion protein Ag4045
• Full Name: chemokine (C-C motif) receptor-like 2
• Calculated Molecular Weight: 344 aa, 40 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC025717
• Gene Symbol: CCRL2
• Gene ID (NCBI): 9034
• RRID: AB_2881971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chemokine CC motif receptor-like 2 (CCRL2) is a seven-transmembrane domain receptor that shares structural and functional similarities with the family of atypical chemokine receptors (ACKRs) (PMID: 28743719). CCRL2 does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 has been found on almost all hemopoietic cells, including monocytes, macrophages, polymorphonuclear neutrophils (PMNs), T cells, dendritic cells, natural killer cells, and CD34+ progenitor cells, with PMNs expressing the highest levels (PMID: 15188357). CCRL2 is also expressed by barrier cells, such as lymphatic and blood endothelial cells and bronchial and intestinal epithelial cells (PMID: 29056935).

Publications

Species	Application	Title
human	WB	Hypertension; Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia; Authors - Lunbo Tan
human	WB	PLoS Pathog; Identification of viral SIM-SUMO2-interaction inhibitors for treating primary effusion lymphoma.; Authors - Ling Ding
human	WB	Onco Targets Ther; Oncogenic Role and Prognostic Value of MicroRNA-937-3p in Patients with Breast Cancer.; Authors - Deyu Li
human	WB	Front Immunol; Identification and validation of chemokine system-related genes in idiopathic pulmonary fibrosis; Authors - Tianming Zhao