CCR7 Polyclonal antibody

CCR7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 25898-1-AP

Synonyms

BLR2, C C chemokine receptor type 7, C C CKR 7, CC CKR 7, CCR 7, CCR7, CD197, CDw197, CMKBR7, EBI1, EVI1, MIP 3 beta receptor

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Validation Data Gallery

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  WB analysis using 25898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25898-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25898-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25898-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25898-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, K-562 cells, C6 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

25898-1-AP targets CCR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCR7 fusion protein Ag22941
• Full Name: chemokine (C-C motif) receptor 7
• Calculated Molecular Weight: 378 aa, 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC035343
• Gene Symbol: CCR7
• Gene ID (NCBI): 1236
• RRID: AB_2880289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-C chemokine receptor type 7 (CCR7), which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probably acts as a mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

Publications

Species	Application	Title
mouse	WB,IF	ACS Nano; Antigen Self-Presented Personalized Nanovaccines Boost the Immunotherapy of Highly Invasive and Metastatic Tumors; Authors - Tingting Wang
rat	IF	Bioact Mater; Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold.; Authors - Tieying Yin
mouse	IHC	Virulence; Intranasal bovine β-defensin-5 enhances antituberculosis immunity in a mouse model by a novel protein-based respiratory mucosal vaccine.; Authors - Zhengmin Liang
rat	IHC	Front Cell Dev Biol; Potential Mechanisms of the Impact of Hepatocyte Growth Factor Gene-Modified Tendon Stem Cells on Tendon Healing.; Authors - Mingzhao Zhang
rat	IF	Stem Cell Res Ther; Tendon stem cell-derived exosomes regulate inflammation and promote the high-quality healing of injured tendon.; Authors - Mingzhao Zhang
human	IHC	Front Mol Biosci; CCR7 Has Potential to Be a Prognosis Marker for Cervical Squamous Cell Carcinoma and an Index for Tumor Microenvironment Change.; Authors - Wei-Jie Tian