Cyclin T1 Polyclonal antibody

Cyclin T1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20992-1-AP

Synonyms

CCNT1, CCNT, CYCT1, Cyclin-T1, Cyclin-T

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Validation Data Gallery

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  WB analysis using 20992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20992-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20992-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20992-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 20992-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20992-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20992-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 20992-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20992-1-AP (Cyclin T1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 20992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin T1 antibody (20992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, Jurkat cells, K-562 cells, Y79 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

20992-1-AP targets Cyclin T1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cyclin T1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: NM_001240
• Gene Symbol: Cyclin T1
• Gene ID (NCBI): 904
• RRID: AB_10950894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

Publications

Species	Application	Title
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB,IF	BMC Cancer; The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study; Authors - Ying E