CCNL1 Polyclonal antibody

CCNL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13138-1-AP

Synonyms

ania 6a, BM 001, CCNL1, Cyclin L, cyclin L1, PRO1073, UNQ530/PRO1073

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Validation Data Gallery

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  WB analysis using 13138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13138-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13138-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13138-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, PC-3 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13138-1-AP targets CCNL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCNL1 fusion protein Ag3731
• Full Name: cyclin L1
• Calculated Molecular Weight: 526 aa, 60 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC007081
• Gene Symbol: CCNL1
• Gene ID (NCBI): 57018
• RRID: AB_10640299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNL1, also named as Cyclin-L1, contains a RS region and belongs to the cyclin family. CCNL1 localizes in the nucleus speckle and is Overexpressed in primary tumors of head and neck squamous cell carcinomas (HNSCC). CCNL1 as transcriptional regulator participates in regulating the pre-mRNA splicing process. It is seems to be involved in the regulation of RNA polymerase II (pol II). CCNL1 functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. It may be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). The calculated molecular weight of CCNL1 is about 55-60 kDa, but we detected a 40-50 kDa isoform by western blotting.