CCNJ Polyclonal antibody

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  WB analysis using 15694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15694-1-AP (CCNJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 15694-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15694-1-AP (CCNJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCNJ Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15694-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Cyclin-J, cyclin J

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15694-1-AP targets CCNJ in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCNJ fusion protein Ag8273
• Full Name: cyclin J
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC043175
• Gene Symbol: CCNJ
• Gene ID (NCBI): 54619
• RRID: AB_3085470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T5M9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNJ (Cyclin-J) Belongs to the Cyclin family. Cyclin J is expressed in early Drosophila embryos, and it is essential for mediating intestinal epithelial cell recovery from bacterial pore-forming toxin attack in both Drosophila and mice. Upon TLR and IFN stimulation, cyclin J restrained macrophage inflammatory responses irrespective of the cell cycle control. Cyclin J interacted with CDKs and facilitated the phosphorylation of a set of CDK substrates, including FoxK1 and Drp1. Phosphorylation inhibited FoxK1-mediated expression of glycolytic genes by reducing FoxK1 nuclear localization. Cyclin J-CDK-dependent Drp1 phosphorylation also resulted in mitochondrial fission and the suppression of ROS production (PMID: 35412849).