CCNI2 Polyclonal antibody

CCNI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18822-1-AP

Synonyms

CCNI2, cyclin I family, member 2, Cyclin I2

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Validation Data Gallery

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  WB analysis using 18822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18822-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18822-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 18822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18822-1-AP (CCNI2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 18822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18822-1-AP (CCNI2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, MCF-7 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

18822-1-AP targets CCNI2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cyclin I family, member 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC144417
• Gene Symbol: CCNI2
• Gene ID (NCBI): 645121
• RRID: AB_10596930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNI2 belongs to the cyclin family.

Publications

Species	Application	Title
mouse	WB,IF	Sci Rep; Cyclin I-like (CCNI2) is a cyclin-dependent kinase 5 (CDK5) activator and is involved in cell cycle regulation.; Authors - Chengcheng Liu