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Cyclin E1 Polyclonal antibody
Cyclin E1 Polyclonal Antibody for FC, IHC, WB, ELISA
Cat no : 11554-1-AP
Validation Data Gallery
|Positive WB detected in
|HT-29 cells, NIH/3T3 cells, mouse heart tissue, HeLa cells, Jurkat cells, human lung tissue, MCF-7 cells, HepG2 cells, K-562 cells, human placenta tissue
|Positive IHC detected in
|mouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|MCF-7 cells, HeLa cells
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:400-1:1600
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11554-1-AP targets Cyclin E1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, zebrafish, pig, Artemia sinica
|Host / Isotype
|Rabbit / IgG
|Cyclin E1 fusion protein Ag2110
|Calculated molecular weight
|410 aa, 47 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).
LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.
Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses.
Shuttling SLC2A4RG is regulated by 14-3-3θ to modulate cell survival via caspase-3 and caspase-6 in human glioma.
J Pineal Res
Mitochondrial cytochrome P450 (CYP) 1B1 is responsible for melatonin-induced apoptosis in neural cancer cells.
Cell Death Dis
α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray