CCNDBP1 Polyclonal antibody

CCNDBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 12363-1-AP

Synonyms

HHM, Grap2 and cyclin-D-interacting protein, GCIP, DIP1, Cyclin-D1-binding protein 1

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Validation Data Gallery

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  WB analysis of HeLa using 12363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12363-1-AP (CCNDBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12363-1-AP (CCNDBP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 12363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12363-1-AP (CCNDBP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12363-1-AP

  Immunofluorescent analysis of Hela cells, using CCNDBP1 antibody 12363-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

12363-1-AP targets CCNDBP1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCNDBP1 fusion protein Ag3032
• Full Name: cyclin D-type binding-protein 1
• Calculated Molecular Weight: 360 aa, 40 kDa
• GenBank Accession Number: BC009689
• Gene Symbol: CCNDBP1
• Gene ID (NCBI): 23582
• RRID: AB_2877849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNDBP1, also named as DIP1, GCIP and HHM, is a helix-loop-helix protein which suppresses tumorigenesis. CCNDBP1 specifically interacts with one of the class III HDAC proteins, SirT6, which is important for maintaining genome stability. CCNDBP1 may negatively regulate cell cycle progression.

Publications

Species	Application	Title
rat	WB	Biomed Pharmacother; Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.; Authors - Ji-Ru Zhang
human	WB,IHC	Front Oncol; CCNDBP1, a Prognostic Marker Regulated by DNA Methylation, Inhibits Aggressive Behavior in Dedifferentiated Liposarcoma via Repressing Epithelial Mesenchymal Transition; Authors - Lingge Yang
human	WB,CoIP	J Cell Mol Med; LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1.; Authors - Yao Liu
human	WB	Oncol Rep; Expression and prognostic significance of TRPV6 in the development and progression of pancreatic cancer.; Authors - He Song
human	WB	Am J Transl Res; Lithium chloride inhibits cell survival, overcomes drug resistance, and triggers apoptosis in multiple myeloma via activation of the Wnt/β-catenin pathway.; Authors - Ruosi Yao
		Oncol Rep; HOXA5 is a tumor suppressor gene that is decreased in gastric cancer.; Authors - Xudong Peng