Cyclin D3 Polyclonal antibody

Cyclin D3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10845-1-AP

Synonyms

CCND3, Cyclin D3

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 10845-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10845-1-AP (CCND3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

10845-1-AP targets Cyclin D3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin D3 fusion protein Ag1292
• Full Name: cyclin D3
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC011616
• Gene Symbol: Cyclin D3
• Gene ID (NCBI): 896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Publications

Species	Application	Title
human	WB	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma
human	WB	FASEB J; C1orf61 acts as a tumor activator in human hepatocellular carcinoma and is associated with tumorigenesis and metastasis.; Authors - Hu Hai-Ming HM
human	WB	J Biol Chem; Sj7170, a unique dual-functional peptide with a specific α-chymotrypsin inhibitory activity and a potent tumor activating effect from scorpion venom.; Authors - Yu Song
human	WB	Phytomedicine; Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action.; Authors - Mingmin Tang
human	WB,IHC	BMC Cancer; PMID: 16978399; Authors - Zhang Ping P
		Int J Oncol; Tetrandrine induces G1/S cell cycle arrest through the ROS/Akt pathway in EOMA cells and inhibits angiogenesis in vivo.; Authors - Wenkai Xiao