Cyclin D2 Polyclonal antibody

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  WB analysis using 10934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10934-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10934-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10934-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10934-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 10934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin D2 antibody (10934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red).

• Featured Product
• KD/KO Validated

Cyclin D2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10934-1-AP

Publications(54)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, ELISA

Synonyms

CCND2, G1/S-specific cyclin-D2, G1/S specific cyclin D2, Cyclin D

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Tested Applications

• Positive WB detected in: Caco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells
• Positive IHC detected in: human renal cell carcinoma tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 1 publications below

Product Information

10934-1-AP targets Cyclin D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin D2 fusion protein Ag1377
• Full Name: cyclin D2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC010958
• Gene Symbol: Cyclin D2
• Gene ID (NCBI): 894
• RRID: AB_2275319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Publications

Species	Application	Title
mouse		Nature; SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.; Authors - Soufiane Boumahdi
human	WB	Cancer Cell; The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.; Authors - Natalia Martinez-Soria ; KD Validated
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
mouse	WB	Dev Cell; Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.; Authors - Yan Gong
mouse		Kidney Int; Transcription factor MafB may play an important role in secondary hyperparathyroidism.; Authors - Naoki Morito
human	IHC	Front Immunol; Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer; Authors - Qianshan Hou