Cyclin B2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Cyclin B2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2G8B7

Cat no : 67726-1-Ig

Synonyms

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

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Validation Data Gallery

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  WB analysis using 67726-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67726-1-Ig

  WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells.

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  IHC staining of human breast cancer using 67726-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67726-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 67726-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse testis using 67726-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 67726-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, K-562 cells, Jurkat cells, HEK-293 cells, U2OS cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67726-1-Ig targets Cyclin B2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin B2 fusion protein Ag16329
• Full Name: cyclin B2
• Calculated Molecular Weight: 398 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC105086
• Gene Symbol: Cyclin B2
• Gene ID (NCBI): 9133
• RRID: AB_2882912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.