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  • KD/KO Validated

Cyclin B2 Polyclonal antibody

Cyclin B2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21644-1-AP


CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

Tested Applications

Positive WB detected inA431 cells, DU 145 cells, rat testis tissue, HeLa cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21644-1-AP targets Cyclin B2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin B2 fusion protein Ag16329
Full Name cyclin B2
Calculated Molecular Weight 398 aa, 45 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC105086
Gene Symbol CCNB2
Gene ID (NCBI) 9133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status. Cyclin B2 can be detected double bands (PMID:1651338).


Product Specific Protocols
WB protocol for Cyclin B2 antibody 21644-1-APDownload protocol
IHC protocol for Cyclin B2 antibody 21644-1-APDownload protocol
IP protocol for Cyclin B2 antibody 21644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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