Cyclin B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 21644-1-AP

Synonyms

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

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Validation Data Gallery

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  WB analysis using 21644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21644-1-AP

  WB result of Cyclin B2 antibody (21644-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected A431 cells.

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  WB analysis of HeLa using 21644-1-AP

  Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis of DU 145 using 21644-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 21644-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21644-1-AP (Cyclin B2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 21644-1-AP

  IP result of anti-Cyclin B2 (IP:21644-1-AP, 4ug; Detection:21644-1-AP 1:1000) with A431 cells lysate 2000ug.

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  IHC staining of human cervical cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP (Cyclin B2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21644-1-AP (Cyclin B2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 21644-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21644-1-AP (Cyclin B2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, DU 145 cells, rat testis tissue, HeLa cells, K-562 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human cervical cancer tissue, human testis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Product Information

21644-1-AP targets Cyclin B2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin B2 fusion protein Ag16329
• Full Name: cyclin B2
• Calculated Molecular Weight: 398 aa, 45 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC105086
• Gene Symbol: Cyclin B2
• Gene ID (NCBI): 9133
• RRID: AB_10755304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status. Cyclin B2 can be detected double bands (PMID:1651338).

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling; Authors - Chaojie Chen
human	WB,IP	EMBO Rep; Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.; Authors - Sikai Liu ; KD Validated
human	WB	Oxid Med Cell Longev; Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation.; Authors - Mei Cao
human	IHC	Elife; Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.; Authors - Xiao-Tong Lin
human	WB	Oncotarget; The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.; Authors - Lifei Fan
human	WB	Ecotoxicol Environ Saf; Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity.; Authors - Liling Su