CCL3 Recombinant antibody

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  IF Staining of RAW 264.7 using 84701-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CCL3 antibody (84701-1-RR, Clone: 241695B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84701-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84701-1-RR against Mouse CCL3 were performed. The affinity constant is 4.00 nM.

CCL3 Recombinant Antibody for IF/ICC, ELISA

Cat No. 84701-1-RR

Clone No.241695B10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Synonyms

Mip1a, Macrophage inflammatory protein 1-alpha, L2G25B, Heparin-binding chemotaxis protein, C-C motif chemokine 3

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84701-1-RR targets CCL3 in IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-C motif) ligand 3
• Calculated Molecular Weight: 10kd
• GenBank Accession Number: NM_011337
• Gene Symbol: Ccl3
• Gene ID (NCBI): 20302
• RRID: AB_3672117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10855
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL3, also known as macrophage inflammatory protein-1 alpha (MIP-1α), is a chemokine that plays a significant role in immune responses. It is involved in recruiting immune cells, particularly monocytes and T cells, to sites of inflammation and infection. CCL3 is produced by various cell types, including activated macrophages and lymphocytes, and is crucial for orchestrating the immune response to infections and tissue injury. In summary, CCL3 is a multifunctional chemokine that is essential for immune cell recruitment and plays a role in various diseases, including chronic inflammatory conditions and cancer.