CCL27 Polyclonal antibody

CCL27 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22301-1-AP

Synonyms

ALP, C C motif chemokine 27, CC chemokine ILC, CCL27, CTACK, CTAK, ESKINE, IL 11 R alpha locus chemokine, ILC, PESKY, SCYA27, Skinkine, Small inducible cytokine A27

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Validation Data Gallery

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  IHC staining of human testis using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 22301-1-AP

  Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 22301-1-AP

  Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human testis tissue, human liver tissue, human liver cancer tissue, human skin tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22301-1-AP targets CCL27 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-C motif) ligand 27
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: NM_006664
• Gene Symbol: CCL27
• Gene ID (NCBI): 10850
• RRID: AB_2879065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL27, also known as CTACK, is a member of the CC chemokine subfamily. CCL27 is predominantly expressed in skin and selectively attracts cutaneous lymphocyte antigen-positive T cells and Langerhans cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. CCL27 is a ligand to CCR10. The binding of CCL27 to CCR10 on T cells promotes their migration to the skin, where they can then participate in local immune responses.