CCL18/MIP-4 Polyclonal antibody

CCL18/MIP-4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22303-1-AP

Synonyms

CCL18, MIP 4, MIP-4, CCL 18, C-C motif chemokine 18

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Validation Data Gallery

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  IHC staining of human tonsillitis using 22303-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22303-1-AP (CCL18/MIP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 22303-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22303-1-AP (CCL18/MIP-4 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 22303-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22303-1-AP (CCL18/MIP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 22303-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22303-1-AP (CCL18/MIP-4 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 22303-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22303-1-AP (CCL18/MIP-4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22303-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22303-1-AP (CCL18/MIP-4 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human heart tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

22303-1-AP targets CCL18/MIP-4 in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• Calculated Molecular Weight: 10 kDa
• GenBank Accession Number: NM_002988
• Gene Symbol: CCL18/MIP-4
• Gene ID (NCBI): 6362
• RRID: AB_2879066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL18 is a small cytokine belonging to the CC chemokine family. The effector functions that CCL18 exerts on the immune system is mainly on the adaptive immune system, while being produced and secreted mainly by innate immune system. Although the functions of CCL18 have been well studied in vitro, physiological effects of CCL18 in vivo have been difficult to characterize because there is no murine analog of CCL18. Only recently has the receptor for CCL18 been identified, and further characterization of CCL18 interactions with its receptor will lead to better understanding of the physiological role of CCL18 in humans.

Publications

Species	Application	Title
human	IHC	ACS Biomater Sci Eng; Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.; Authors - Binghui Liu
human	IHC	Front Immunol; Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction; Authors - Hang Ji
human	IHC	J Cell Physiol; Surgical trauma-induced CCL18 promotes recruitment of regulatory T cells and colon cancer progression.; Authors - Zhirong Sun
human	IF	Nat Commun; Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis; Authors - Shu Zhang
human	IF	Med; Multimodal single-cell analyses reveal mechanisms of perianal fistula in diverse patients with Crohn's disease; Authors - Rachel M Levantovsky
human	IHC	J Allergy Clin Immunol; ALOX15+ M2 macrophages contribute to epithelial remodeling in eosinophilic chronic rhinosinusitis with nasal polyps; Authors - Chang Liu