CCL17 Polyclonal antibody

CCL17 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22342-1-AP

Synonyms

A 152E5.3, ABCD 2, C C motif chemokine 17, CC chemokine TARC, CCL17, SCYA17, Small inducible cytokine A17, TARC

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Validation Data Gallery

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  IHC staining of human colon using 22342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22342-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22342-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22342-1-AP (CCL17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon tissue, human placenta tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

The immunogen of 22342-1-AP is CCL17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCL17 fusion protein Ag17832
• Full Name: chemokine (C-C motif) ligand 17
• Calculated Molecular Weight: 94 aa, 11 kDa
• GenBank Accession Number: BC112066
• Gene Symbol: CCL17
• Gene ID (NCBI): 6361
• RRID: AB_2879081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL17, also named as TARC, is a member of the CC chemokine family, and is highly expressed by thymus and other cells, including keratinocytes, endothelial cells, dendritic cells, bronchial epithelial cells and fibroblasts. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 induced chemotaxis and Ca2+ influx in the cells stably expressing CCR4, thereby demonstrating the ligand-receptor relationship between CCL17 and CCR4.

Publications

Species	Application	Title
human	IHC	Br J Haematol; Expression of multiple cytokines and chemokine receptor 4 in angioimmunoblastic T-cell lymphoma with abundant plasma cell infiltration involving the skin.; Authors - Shoko Nakayama
human	IHC	Hemasphere; NOTCH1 fusions in pediatric T-cell lymphoblastic lymphoma: A high-risk subgroup with CCL17 (TARC) levels as diagnostic biomarker; Authors - Emma Kroeze