CCK Polyclonal antibody

CCK Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

3

Cat no : 13074-2-AP

Synonyms

CCK, CCK12 Cholecystokinin 8, CCK18 Cholecystokinin 12, CCK25 Cholecystokinin 18, CCK33 Cholecystokinin 25, CCK39 Cholecystokinin 33, CCK5, CCK7 Cholecystokinin 5, CCK8 Cholecystokinin 7, cholecystokinin

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Validation Data Gallery

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  IHC staining of human pancreas using 13074-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13074-2-AP (CCK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 13074-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13074-2-AP (CCK Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 13074-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13074-2-AP (CCK Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13074-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13074-2-AP (CCK Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human pancreas tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13074-2-AP targets CCK in IF, IHC, ELISA, Cell treatment applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCK fusion protein Ag3725
• Full Name: cholecystokinin
• Calculated Molecular Weight: 115 aa, 13 kDa
• GenBank Accession Number: BC008283
• Gene Symbol: CCK
• Gene ID (NCBI): 885
• RRID: AB_2228728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cholecystokinin (CCK) is a classic gut hormone. CCK is also a complex system of peptides expressed in several molecular forms in enteroendocrine I cells, in cerebral and peripheral neurons, in cardiac myocytes and spermatozoa. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the peptide hormones cholecystokinin-8, -12, -33, and others. The encoded peptides have been shown to regulate gastric acid secretion and food intake.

Publications

Species	Application	Title
	Cell treatment	J Fungi (Basel); Two Cladosporium Fungi with Opposite Functions to the Chinese White Wax Scale Insect Have Different Genome Characters.; Authors - Wei Liu
		J Ethnopharmacol; Tibetan medicine Ru-yi-Zhen-bao Pills exhibits anti-migraine effect through mediating PAG anti-nociceptive channel.; Authors - Ya Min Luo
	IF	Sci Total Environ; Micro/nanoplastics impair the feeding of goldfish by disrupting the complicated peripheral and central regulation of appetite; Authors - Weixia Zhang