CCDC91 Polyclonal antibody

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  WB analysis using 31097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31097-1-AP (CCDC91 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 31097-1-AP

  WB result of CCDC91 antibody (31097-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC91 transfected HeLa cells.

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  IF Staining of U2OS using 31097-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCDC91 antibody (31097-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

CCDC91 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31097-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

p56, HSD8, GGABP, GGA-binding partner, Coiled-coil domain-containing protein 91

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells, rat testis tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31097-1-AP targets CCDC91 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC91 fusion protein Ag34863
• Full Name: coiled-coil domain containing 91
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC028682
• Gene Symbol: CCDC91
• Gene ID (NCBI): 55297
• RRID: AB_3669854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z6B0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC91, also known as GGABP, p56, HSD8, is a critical accessory protein that promotes transport of carrier vesicles between the Golgi and lysosomes and lysosomal enzyme maturation (PMID: 17596511).