CCDC72 Polyclonal antibody

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  WB analysis of A549 using 20393-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 20393-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20393-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 20393-1-AP

  IP result of anti-CCDC72 (IP:20393-1-AP, 4ug; Detection:20393-1-AP 1:500) with A549 cells lysate 2400 ug.

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  IHC staining of human skin using 20393-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 20393-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 20393-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCDC72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20393-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Translation machinery-associated protein 7, TMA7, HSPC330, HSPC016, Coiled-coil domain-containing protein 72

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, MCF-7 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

20393-1-AP targets CCDC72 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC72 fusion protein Ag13792
• Full Name: coiled-coil domain containing 72
• Calculated Molecular Weight: 64 kDa, 7 kDa
• Observed Molecular Weight: 7 kDa
• GenBank Accession Number: BC001102
• Gene Symbol: CCDC72
• Gene ID (NCBI): 51372
• RRID: AB_10667404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2S6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC72, also named as TMA7, is a novel modulator for hair follicle development. CCDC72 has the promoter activity and regulation of gene expression.

Publications

Species	Application	Title
human	WB,IHC	Int J Biol Sci; IGF2BP3 Regulates TMA7-mediated Autophagy and Cisplatin Resistance in Laryngeal Cancer via m6A RNA Methylation; Authors - Like Yang
mouse	WB	bioRxiv; Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion; Authors - Shiyu Luo
mouse	WB	Dis Model Mech; HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells; Authors - Shiyu Luo