CCDC6 Monoclonal antibody, PBS Only

CCDC6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6A8

Cat no : 67637-1-PBS

Synonyms

CCDC6, D10S170, FLJ32286, H4, Protein H4, PTC, TPC, TST1

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Validation Data Gallery

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  WB analysis using 67637-1-Ig (same clone as 67637-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67637-1-Ig (same clone as 67637-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 67637-1-Ig (same clone as 67637-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67637-1-Ig (same clone as 67637-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CCDC6 antibody (67637-1-Ig, Clone: 1D6A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

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Product Information

67637-1-PBS targets CCDC6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCDC6 fusion protein Ag6952
• Full Name: coiled-coil domain containing 6
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC036757
• Gene Symbol: CCDC6
• Gene ID (NCBI): 8030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CCDC6 (Coiled-coil domain-containing protein 6) is also named as Protein H4, D10S170 and TST1. CCDC6, was initially isolated as part of a tumorigenic DNA originated by the fusion of CCDC6 with the tyrosine kinase of RET receptor. CCDC6 has been considered as an accidental partner of the RET protooncogene, providing the promoter and the first 101 aa necessary for the constitutive activation of the oncogenic Tyrosine Kinase (TK) RET in thyroid cells. The 65 kDa product of CCDC6 has a nuclear transfer sequence with no transmembrane domains and is predicted to locate in both the nucleus and the cytoplasm (PMID: 29044514). The CCDC6 is a phosphoprotein, predicted target of several S/T kinases which can modulate the protein stability and the intracellular shuttling into the nucleus upon different cellular signals mediated by ERK1/2, ATM and CDK1/2. CCDC6 is involved in cellular response to DNA damage mediated by ATM, with the final result of promoting cellular apoptosis. CCDC6 depleted cells are considered defective of DNA repair checkpoint and proceed faster than the control cells in the cell cycle upon induced DNA damage (PMID: 22655027).